NAD+ is a pivotal coenzyme found in all living cells, functioning as a primary electron carrier in various metabolic pathways. In laboratory research, it is utilized to study the kinetics of redox enzymes and the biochemical mechanisms of cellular respiration.
Technical Profile
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Chemical Name: Nicotinamide adenine dinucleotide
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Molecular Formula: $C_{21}H_{27}N_{7}O_{14}P_{2}$
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Molecular Weight: 663.43 g/mol
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Purity: ≥98% (Enzymatic/HPLC)
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Form: Lyophilized powder
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Storage: Store at -20°C for long-term stability; hygroscopic (keep away from moisture).
Laboratory Applications
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Redox Titration: Used as a substrate for evaluating the activity of various oxidoreductase enzymes.
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Cofactor Signaling: Investigating the enzymatic activity of sirtuins and PARPs in in vitro molecular assays.
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Metabolic Mapping: Observing the conversion rates between the oxidized (NAD+) and reduced (NADH) states in isolated biochemical systems.
